benjjneb / dada2
git clone https://github.com/benjjneb/dada2.git
v1.26 · 3y ago
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Accurate sample inference from amplicon data with single nucleotide resolution
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LanguageR
LicenseLGPL-3.0
Created2014-12-17 (11y ago)
Last push2mo ago
Watchers37
OwnerUser
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- GitHub: github.com/benjjneb/dada2
- Homepage: http://benjjneb.github.io/dada2/
- Releases · Issues · Hacker News mentions
README excerpt
# dada2 Exact sample inference from high-throughput amplicon data. Resolves real variants differing by as little as one nucleotide. Visit the DADA2 website for the most detailed and up-to-date documentation. ### Installation The dada2 package binaries are available through Bioconductor: [code] In order to install dada2 from source (and get the latest and greatest new features) see our installation from source instructions. ### Documentation The tutorial walkthrough of the DADA2 pipeline on paired end Illumina Miseq data. The dada2 R package manual. Further documentation is available on the DADA2 front page. ### DADA2 Articles DADA2: High resolution sample inference from Illumina amplicon data. Nature Methods, 2016. (Open Access link.) Bioconductor workflow for microbiome data analysis: from raw reads to community analyses. F1000 Research, 2016. Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. ISMEJ, 2017. High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution. Nucleic Acids Research, 2019. ### Other Resources Planned feature improvements are publicly catalogued at the main DADA2 development site on github, specifically on the "Issues" page for DADA2: https://github.com/benjjneb/dada2/issues If the feature you are hoping for is not listed, you are welcome to add it as a feature request "issue" on this page. This request will be publicly available and listed on the page. Bugs and difficulties in using DADA2 are also welcome on the issue tracker.