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benjjneb / dada2

git clone https://github.com/benjjneb/dada2.git v1.26 · 3y ago 🔥 on GitHub Trending today

Accurate sample inference from amplicon data with single nucleotide resolution

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Star history (as recorded here)

2026-10-20 · 559 ★ 2026-10-23 · 559 ★
LanguageR
LicenseLGPL-3.0
Created2014-12-17 (11y ago)
Last push2mo ago
Watchers37
OwnerUser

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  • 2026-10-23 — #3 daily in r
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README excerpt
# dada2
Exact sample inference from high-throughput amplicon data. Resolves real variants differing by as little as one nucleotide. Visit the DADA2 website for the most detailed and up-to-date documentation.
### Installation
The dada2 package binaries are available through Bioconductor:
 [code] 
In order to install dada2 from source (and get the latest and greatest new features) see our installation from source instructions.
### Documentation
The tutorial walkthrough of the DADA2 pipeline on paired end Illumina Miseq data. 
The dada2 R package manual.
Further documentation is available on the DADA2 front page. 
### DADA2 Articles
DADA2: High resolution sample inference from Illumina amplicon data. Nature Methods, 2016. (Open Access link.)
Bioconductor workflow for microbiome data analysis: from raw reads to community analyses. F1000 Research, 2016.
Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. ISMEJ, 2017.
High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution. Nucleic Acids Research, 2019.
### Other Resources
Planned feature improvements are publicly catalogued at the main DADA2 development site on github, specifically on the "Issues" page for DADA2:
https://github.com/benjjneb/dada2/issues
If the feature you are hoping for is not listed, you are welcome to add it as a feature request "issue" on this page. This request will be publicly available and listed on the page.
Bugs and difficulties in using DADA2 are also welcome on the issue tracker.